Analytical tools for single-molecule fluorescence imaging in cellulo
نویسندگان
چکیده
منابع مشابه
Analytical tools for single-molecule fluorescence imaging in cellulo.
Recent technological advances in cutting-edge ultrasensitive fluorescence microscopy have allowed single-molecule imaging experiments in living cells across all three domains of life to become commonplace. Single-molecule live-cell data is typically obtained in a low signal-to-noise ratio (SNR) regime sometimes only marginally in excess of 1, in which a combination of detector shot noise, sub-o...
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Single-molecule fluorescence imaging is a powerful tool that enables real-time observation of DNA-protein or RNA-protein interactions with a nanometer precision. Here, we provide a detailed procedure for a previously developed single-molecule fluorescence method, termed "single-molecule protein-induced fluorescence enhancement" (smPIFE). While smFRET (Förster resonance energy transfer) requires...
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Observability of individual fluorescent molecules under a microscope has many applications in biology.1, 2 In particular, by grafting fluorophores to biomolecules, they can be tracked in real time in live cells for monitoring of their conformational changes and interactions with other molecules. In other words, singlemolecule imaging allows scientists to read the textbook of life pretty much as...
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Single-molecule fluorescence microscopy enables non-invasive, high-sensitivity, high-resolution imaging, and this direct, quantitative method has recently been extended to understanding organization, dynamics and cooperativity of macromolecules in prokaryotes. In this issue of Molecular Microbiology, Bakshi et al. (2012) examine fluorescently labelled ribosomes and RNA polymerase (RNAP) in live...
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Common uorescent agents include organic uorophores (e.g., Cy5), uorescent proteins (e.g., GFP), and semiconductor quantum dots each with different size, photobleaching, blinking, and labeling ef ciency properties. Wideeld detection with EMCCD camera enables parallel measurements. The temporal resolution is >ms, and the working concentration is limited to nM or less. Confocal detection from an e...
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ژورنال
عنوان ژورنال: Phys. Chem. Chem. Phys.
سال: 2014
ISSN: 1463-9076,1463-9084
DOI: 10.1039/c4cp00219a